LOCUS       Exported                4008 bp ds-DNA    circular SYN 10-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0050max_producer_luxI
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Daniel Gerngross
  TITLE     piG0050max
  JOURNAL   Exported 10 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        50..84
                     /note="const. promoter (J23100)"
     RBS             105..109
                     /note="RBS (B0034)"
     CDS             117..701
                     /codon_start=1
                     /note="luxI (C0161)"
                     /translation="MTIMIKKSDFLAIPSEEYKGILSLRYQVFKQRLEWDLVVENNLES
                     DEYDNSNAEYIYACDDTENVSGCWRLLPTTGDYMLKSVFPELLGQQSAPKDPNIVELSR
                     FAVGKNSSKINNSASEITMKLFEAIYKHAVSQGITEYVTVTSTAIERFLKRIKVPCHRI
                     GDKEIHVLGDTKSVVLSMPINEQFKKAVLN*"
     terminator      713..799
                     /gene="Escherichia coli rrnB"
                     /note="rrnB T1 terminator"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      914..1685
                     /note="pBBR1 oriV"
                     /note="replication origin of the broad-host-range plasmid 
                     pBBR1 from Bordetella bronchiseptica; requires the pBBR1 
                     Rep protein for replication"
     CDS             1686..2348
                     /codon_start=1
                     /product="replication protein for the broad-host-range 
                     plasmid pBBR1 from Bordetella bronchiseptica"
                     /note="pBBR1 Rep"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLLH
                     HLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAYVV
                     NDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGPGEE
                     PPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLPRR"
     oriT            2359..2467
                     /note="incP origin of transfer"
     CDS             complement(2613..3803)
                     /note="TetR"
     terminator      3910..4004
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
ORIGIN
        1 actagtcgcc agggttttcc cagtcacgac gcggccgcaa gcttgcatgt tgacggctag
       61 ctcagtccta ggtacagtgc tagcagcgaa acacgcatat tttaaggagg tactacatga
      121 ctataatgat aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc
      181 taagtcttcg ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata
      241 accttgaatc agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata
      301 ctgaaaatgt aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa
      361 gtgtttttcc tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat
      421 taagtcgttt tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta
      481 caatgaaact atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg
      541 taacagtaac atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc
      601 gtattggaga caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc
      661 ctattaatga acagtttaaa aaagcagtct taaattaata aattaaaggc atcaaataaa
      721 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      781 tctcctgagt aggacaaatc cgccgcccta gacagctggg cgcgcccccc ctacgggctt
      841 gctctccggg cttcgccctg cgcggtcgct gcgctccctt gccagcccgt ggatatgtgg
      901 acgatggccg cgagcggcca ccggctggct cgcttcgctc ggcccgtgga caaccctgct
      961 ggacaagctg atggacaggc tgcgcctgcc cacgagcttg accacaggga ttgcccaccg
     1021 gctacccagc cttcgaccac atacccaccg gctccaactg cgcggcctgc ggccttgccc
     1081 catcaatttt tttaattttc tctggggaaa agcctccggc ctgcggcctg cgcgcttcgc
     1141 ttgccggttg gacaccaagt ggaaggcggg tcaaggctcg cgcagcgacc gcgcagcggc
     1201 ttggccttga cgcgcctgga acgacccaag cctatgcgag tgggggcagt cgaagggcga
     1261 agcccgcccg cctgcccccc gagcctcacg gcggcgagtg cgggggttcc aagggggcag
     1321 cgccaccttg ggcaaggccg aaggccgcgc agtcgatcaa caagccccgg aggggccact
     1381 ttttgccgga gggggagccg cgccgaaggc gtgggggaac cccgcagggg tgcccttctt
     1441 tgggcaccaa agaactagat atagggcgaa atgcgaaaga cttaaaaatc aacaacttaa
     1501 aaaagggggg tacgcaacag ctcattgcgg caccccccgc aatagctcat tgcgtaggtt
     1561 aaagaaaatc tgtaattgac tgccactttt acgcaacgca taattgttgt cgcgctgccg
     1621 aaaagttgca gctgattgcg catggtgccg caaccgtgcg gcacccctac cgcatggaga
     1681 taagcatggc cacgcagtcc agagaaatcg gcattcaagc caagaacaag cccggtcact
     1741 gggtgcaaac ggaacgcaaa gcgcatgagg cgtgggccgg gcttattgcg aggaaaccca
     1801 cggcggcaat gctgctgcat cacctcgtgg cgcagatggg ccaccagaac gccgtggtgg
     1861 tcagccagaa gacactttcc aagctcatcg gacgttcttt gcggacggtc caatacgcag
     1921 tcaaggactt ggtggccgag cgctggatct ccgtcgtgaa gctcaacggc cccggcaccg
     1981 tgtcggccta cgtggtcaat gaccgcgtgg cgtggggcca gccccgcgac cagttgcgcc
     2041 tgtcggtgtt cagtgccgcc gtggtggttg atcacgacga ccaggacgaa tcgctgttgg
     2101 ggcatggcga cctgcgccgc atcccgaccc tgtatccggg cgagcagcaa ctaccgaccg
     2161 gccccggcga ggagccgccc agccagcccg gcattccggg catggaacca gacctgccag
     2221 ccttgaccga aacggaggaa tgggaacggc gcgggcagca gcgcctgccg atgcccgatg
     2281 agccgtgttt tctggacgat ggcgagccgt tggagccgcc gacacgggta acgctgccgc
     2341 gccggtaggg ccggcctacg gccagcctcg cagagcagga ttcccgttga gcaccgccag
     2401 gtgcgaataa gggacagtga agaaggaaca cccgctcgcg ggtgggccta cttcacctat
     2461 cctgcccggc tgacgccgtt ggatacacca aggaaagtct acacgaaccc tttggcaaaa
     2521 tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa atcgctataa tgaccccgaa
     2581 gcagggttat gcagcggaaa aggacaattg tctcaggtcg aggtggcccg gctccatgca
     2641 ccgcgacgca acgcggggag gcagacaagg tatagggcgg cgcctacaat ccatgccaac
     2701 ccgttccatg tgctcgccga ggcggcataa atcgccgtga cgatcagcgg tccagtgatc
     2761 gaagttaggc tggtaagagc cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct
     2821 acctgcctgg acagcatggc ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga
     2881 atcataatgg ggaaggccat ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc
     2941 gcgtcggccg ccatgccggc gataatggcc tgcttctcgc cgaaacgttt ggtggcggga
     3001 ccagtgacga aggcttgagc gagggcgtgc aagattccga ataccgcaag cgacaggccg
     3061 atcatcgtcg cgctccagcg aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc
     3121 acctgtccta cgagttgcat gataaagaag acagtcataa gtgcggccac aatggtcatg
     3181 ccccgcgccc accggaagga gctgactggg ttgaaggctc tcaagggcat cggacggcgc
     3241 tctcccttat gcgactcctg cattaggaag cagcccagta gtaggttgag gccgttgagc
     3301 accgccgccg caaggaatgg tgcgtgcagg gagatggcgc ccaacagtcc cccggccacg
     3361 gggcctgcca ccatacccac gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga
     3421 tcttccccat cggtgatgtc ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg
     3481 atgccggcca cgatgcgtcc ggcgtagaga atccacagga cgggtgtggt cgccatgatc
     3541 gcgtagtcga tagtggctcc aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg
     3601 tcggacagtg ctccgagaac gggtgcgcat agaaattgca tcaacgcata tagcgctagc
     3661 agcacgccat agtgactggc gatgctgtcg gaatggacga tatcccgcaa gaggcccggc
     3721 agtaccggca taaccaagcc tatgcctaca gcatccaggg tgacggtgcc gaggatgacg
     3781 atgagcgcat tgttagattt catacacggt gcctgactgc gttagcaatt taactgtgat
     3841 aaactaccgc attacagttt atcgatgata agctgtcaaa tttaaatcgt aattattggg
     3901 gacccctgga ttctcaccaa taaaaaacgc ccggcggcaa ccgagcgttc tgaacaaatc
     3961 cagatggagt tctgaggtca ttactggatc tatcaacagg agtccaag
//
